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Table 4 Human sperm penetration into the ZP of unfertilised and fertilised oocytes

From: Zona pellucida from fertilised human oocytes induces a voltage-dependent calcium influx and the acrosome reaction in spermatozoa, but cannot be penetrated by sperm

  Unfertilised oocytes Fertilised oocytes
Experiment Number of embedded spermatozoa per ZP mean ± SEM Penetrated zona pellucida % Number of embedded spermatozoa per ZP mean ± SEM Penetrated zona pellucida %
1 0.93 ± 0.32 57 (8/14)a 0.18 ± 0.13** 18* (2/11)
2 1.14 ± 0.60 57 (4/7) 0.22 ± 0.11** 17*(4/23)
3 1.14 ± 0.43 57 (8/14) 0.26 ± 0.13** 21*(4/19)
Total 1.06 ± 0.23 57 (20/35) 0.23 ± 0.07*** 19*** (10/53)
  1. ()anumber of oocytes with penetrated zona pellucida/total number of oocytes; *,**,*** statistically different from unfertilised oocytes: * p < 0.05; ** p < 0.01; *** p < 0.001 with Student's t test (mean ± SEM) or Chi-squared test (%).