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Table 3 Effect of solubilised human zona pellucida providing from fertilised and unfertilised oocytes on sperm Ca2+ concentration and acrosome reaction rate

From: Zona pellucida from fertilised human oocytes induces a voltage-dependent calcium influx and the acrosome reaction in spermatozoa, but cannot be penetrated by sperm

  

[Ca2+] increase pattern a

[Ca2+]i (nM)

 

Oocytes

Treatment

I

II

Basal level

Peakb

Sustained phase

AR (%)

Unfertilised (n = 9)

hZP

4

5

448.5 ± 50.4

1012.2 ± 229.7*

1171.7 ± 262.4*

60.7 ± 9.1**

 

Control

  

383.3 ± 44.1

423.3 ± 41.8

448.8 ± 49.2

21.8 ± 2.7

Fertilised (n = 12)

hZP

8

4

416.0 ± 48.5

902.5 ± 165.9**

1050.4 ± 217.4*

61.6 ± 6.2***

 

Control

  

389.2 ± 45.9

378.7 ± 46.6

395.0 ± 38.0

20.7 ± 2.7

  1. The results are expressed as mean ± SEM; The number of experiment is shown in brackets (n =); [Ca2+]I = intracellular Ca2+ concentration; AR = acrosome reactiona Number of sperm samples displaying pattern I or II increases in calcium concentration, as shown in Figure 1; b peak was calculated only for sperm samples displaying pattern 1; *, **, *** statistically different from the corresponding control: * p < 0.05, ** p < 0.01,*** p < 0.001 with Student's t test.