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Table 3 Effect of solubilised human zona pellucida providing from fertilised and unfertilised oocytes on sperm Ca2+ concentration and acrosome reaction rate

From: Zona pellucida from fertilised human oocytes induces a voltage-dependent calcium influx and the acrosome reaction in spermatozoa, but cannot be penetrated by sperm

   [Ca2+] increase pattern a [Ca2+]i (nM)  
Oocytes Treatment I II Basal level Peakb Sustained phase AR (%)
Unfertilised (n = 9) hZP 4 5 448.5 ± 50.4 1012.2 ± 229.7* 1171.7 ± 262.4* 60.7 ± 9.1**
  Control    383.3 ± 44.1 423.3 ± 41.8 448.8 ± 49.2 21.8 ± 2.7
Fertilised (n = 12) hZP 8 4 416.0 ± 48.5 902.5 ± 165.9** 1050.4 ± 217.4* 61.6 ± 6.2***
  Control    389.2 ± 45.9 378.7 ± 46.6 395.0 ± 38.0 20.7 ± 2.7
  1. The results are expressed as mean ± SEM; The number of experiment is shown in brackets (n =); [Ca2+]I = intracellular Ca2+ concentration; AR = acrosome reactiona Number of sperm samples displaying pattern I or II increases in calcium concentration, as shown in Figure 1; b peak was calculated only for sperm samples displaying pattern 1; *, **, *** statistically different from the corresponding control: * p < 0.05, ** p < 0.01,*** p < 0.001 with Student's t test.