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Figure 2 | BMC Developmental Biology

Figure 2

From: Zona pellucida from fertilised human oocytes induces a voltage-dependent calcium influx and the acrosome reaction in spermatozoa, but cannot be penetrated by sperm

Figure 2

Sperm binding test using unfertilised and fertilised human oocytes. Unfertilised human oocytes recovered after IVF failure (A) and fertilised oocytes with a high fragmentation rate two days after fertilisation (B), were cleared of bound spermatozoa and re-inseminated. After three hours, oocytes were similarly washed by repeated aspiration in a pipette, and the number of spermatozoa bound to the surface was determined under a microscope. Original magnification ×400.

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