Figure 3

Increase of apoptosis in the Necdin mutant DRGs. (A) Time course of TUNEL staining in lumbar DRGs. Shown are the box-plots describing the number of TUNEL positive cells for lumbar DRGs; statistical comparisons were made using the Mann-Whitney test; asterisks show differences that are statistically significant (*, p < 0.05). (B) Double immunofluorescence labelling for TUNEL (in red) and Neurofilament (in green) in a lumbar ganglion of an E12.5 mutant embryo. In this figure, one immunopositive-cell for Neurofilament in the field whose nuclei are labelled by the apoptosis detection method (arrow). (C) Double-labelling for TUNEL (in red) and BrdU (in green) after 2 hr injection; co-labelling was never observed, indicating that precursors are not dying in the Necdin mutant animals. Scale bar: 10 μm (B and C).