Figure 1

Expression of Necdin in DRGs through development. (A) Ndn in situ hybridization (ISH) on sagittal section at E13.5 displays expression in the central and peripheral nervous system, in particular in the forebrain (fb), the midbrain (mb), the hindbrain (hb) and in the DRGs (arrows). (B, C) Ndn mRNA is detected in developing sensory neurons at E11.5 (B) and E13.5 (C). At E12.5, Necdin protein is not expressed in progenitor cells but in post-mitotic neurons. (D) Sections through lumbar DRGs of E12.5 embryos co-labelled with anti-Necdin antibody (in red) and with anti-Neurofilament antibody (in green) reveal a co-localisation between both staining (in yellow). (E) Consecutive sections double-labelled with anti-Necdin antibody (in red) and anti-BrdU (in green) show no co-localisation of both markers. (F) Magnification of a lumbar DRG at E13.5 labelled by immunohistochemistry using anti-Necdin antibody in combination with Hæchst, to look at the subcellular localisation of Necdin. Necdin is detected in the cytoplasm and nucleus of cells. Arrowheads indicate examples of both weak and strong expression level of Necdin in sensory neurons that are readily observable at E13.5. Scale bar: 200 μm (A); 50 μm (B and C); 100 μm (D, E and F).