Figure 1From: Temporal and spatial control of transgene expression using laser induction of the hsp70promoterCreating and testing the transgenic line of butterflies. (a) Plasmid illustration of piggyBac [3xP3-DsRed, hsp70-EGFP] (b) Eye of a wild-type B. anynana adult versus (c) eye of a transgenic individual showing DsRed expression. Scale bar = 500 μm. (d) Southern blot of EcoRI digests of wild-type (wt) genomic DNA, J3 transgenic line genomic DNA and pBac{3xP3-DsRed, Hsp70-EGFP} plasmid. Blot was probed for DsRed PCR fragment. J3 transgenic line is carrying a single insertion of the piggyBac element. (e, f) Transgenic first instar larva before and after heat shock. (g, h) Wild-type first instar larva before and after heat shock. (i, j) Transgenic pupa before and after heat shock. (k, l) Wild-type pupa before and after heat shock. Scale bar = 2 mm.Back to article page