Figure 2
From: Comparative insights into questions of lepidopteran wing pattern homology
pSmad expression in Bicyclus anynana larval and pupal wings. (A, B) Joint visualization of pSmad (red) and En (green) in late 5th instar larval wing discs (50×); (A) pSmad is present along the margin but is absent in the future eyespot centers (arrowhead) where En (B) is visible; (C) pSmad and En (D) are present in the future eyespot centers, at 12 h after pupation. Note the patchiness of the pSamad staining outside of the focal area, perhaps indicating centers of epidermal cell growth (100×); (E) pSmad is no longer visible in the eyespot center (arrowhead) at 18 h after pupation, whereas en expression (F) extends to a ring pattern of scale-building cells surrounding the focus (200×).