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Figure 5 | BMC Developmental Biology

Figure 5

From: Functional role of aspartic proteinase cathepsin D in insect metamorphosis

Figure 5

Internucleosomal DNA fragmentation of the larval gut in RNAi-mediated B. mori pupae. BmCatD dsRNA was injected into pupae on day 1 (B), 5 (C) or both 1 and 5 (D) of the pupal stage, respectively. The control was the untreated pupae (A). DNA was extracted from the gut on day 1, 3, 5, 7, and 10 of the pupal stage (as indicated), respectively. DNA fragmentation was assessed by 1.0% agarose gel electrophoresis (upper). Total RNA from larval gut was extracted as above. The expression level of BmCatD mRNA was analyzed by Northern blot analysis (lower).

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