Figure 5From: Functional role of aspartic proteinase cathepsin D in insect metamorphosisInternucleosomal DNA fragmentation of the larval gut in RNAi-mediated B. mori pupae. BmCatD dsRNA was injected into pupae on day 1 (B), 5 (C) or both 1 and 5 (D) of the pupal stage, respectively. The control was the untreated pupae (A). DNA was extracted from the gut on day 1, 3, 5, 7, and 10 of the pupal stage (as indicated), respectively. DNA fragmentation was assessed by 1.0% agarose gel electrophoresis (upper). Total RNA from larval gut was extracted as above. The expression level of BmCatD mRNA was analyzed by Northern blot analysis (lower).Back to article page