Figure 2
From: Functional role of aspartic proteinase cathepsin D in insect metamorphosis
BmCatD expression profile and internucleosomal DNA fragmentation in the fat body and gut of B. mori. (A) Relative mRNA levels of BmCatD during the fifth larval instar to pupal stage. The level of BmCatD mRNA is calculated relative to that of the background (shown as 0%). The fat body and gut of B. mori were collected during the fifth larval instar to pupal stage (as indicated), respectively. (B, C) BmCatD expression and internucleosomal DNA fragmentation in the fat body (B) and gut (C) of B. mori. The expression level of BmCatD mRNA (upper) and its protein (middle) was analyzed by Northern blot and Western blot analyses, respectively. Internucleosomal DNA fragmentation in fat body and gut cells was assessed by 1.0% agarose gel electrophoresis (lower).