Table 1 Primers used for PCR reactions. The sequences of the primers used to amplify PDE3A and β-actin mRNA in the oocyte cDNA are shown, along with the expected sizes of the PCR products. Sasseville et al. 2006.
From: New insight into the role of phosphodiesterase 3A in porcine oocyte maturation
Gene | Primer | Primer sequences | PCR product expected size (bp) |
|---|---|---|---|
β-actin | β-actin-F | 5'-ATCCTGACCCTCAAGTACCCCAT-3' | 242 |
β-actin-R | 5'-TACTCCTGCTTGCTGATCCACAT-3' | ||
PDE3A | PDE3A-F | 5'-GAACAGATGACACTGCTCAAGTT-3' | 180 |
PDE3A-R | 5'-GAGCAAGAATTGGTTTGTCCAG-3' |