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Figure 9 | BMC Developmental Biology

Figure 9

From: New insight into the role of phosphodiesterase 3A in porcine oocyte maturation

Figure 9

Effects of specific PDE3 inhibition on maturation of OA-treated oocytes over 48 hours of IVM. COC were initially cultured either in control conditions (A-C-E) or in okadaic acid (2 μM)-supplemented medium for 3 hours (B-D-F) Each group was cultured up to 12, 24, 36 and 48 hours of IVM alone (open symbols) or with cilostamide (20 μM, closed symbols). The nuclear status was then assessed in a sample of oocytes from each condition every 12 hours of IVM up to 48 hours. Results are presented as the mean percentages of oocytes in GV arrest (A-B), intermediate stage (C-D) and mature stage (E-F) ± SEM of at least three replicates. A total of 845 oocytes were used. Asterisks indicate significant differences between control and cilostamide treated-oocytes or between okadaic acid and okadaic acid-cilostamide-treated oocytes according to the Bonferroni post hoc test. (*, P < 0.05; **, P < 0.01; ***, P < 0.001).

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