Figure 8

Effects of specific PDE3 inhibition on maturation of OA-treated oocytes. COC were cultured in okadaic acid (2 μM)-supplemented medium for 3 hours followed by culture in either inhibitor-free or cilostamide (20 μM)-supplemented medium for up to 24 hours of IVM. The nuclear status of the oocytes was then assessed. Each column represents the mean percentages of oocytes in GV arrest (open bars), intermediate stage (grey bars) and mature stage (closed bars) ± SEM of at least three replicates. The number of oocytes per treatment is indicated under each bar. Statistical analysis by ANOVA showed that the treatments had significant effects (P < 0.0001). Different letters indicate significant differences (P < 0.05) according to the Bonferroni post hoc test.