Figure 4From: New insight into the role of phosphodiesterase 3A in porcine oocyte maturationEffect of specific PDE3 inhibitor, cilostamide, on oocyte maturation. COC were cultured first in cilostamide-free IVM medium for 19 hours followed by the addition of cilostamide (20 μM) for up to 48 hours in culture. Control (--■--) and cilostamide-treated (---▲---) experiments show the percentages of oocytes in GV arrest (A), intermediate stage (B) and mature stage (C). Values represent the means ± SEM of at least three replicates. A total of 590 oocytes were used. Statistical analysis by ANOVA showed that effects of treatments were significant (P = 0.001). Asterisks indicate significant differences according to the Bonferroni post hoc test (*, P < 0.05; **, P < 0.01; ***, P < 0.001).Back to article page