Figure 3

Time-dependent ability of specific PDE3 inhibitionto maintain COC in meiotic arrest. (A) COC were cultured in cilostamide-free medium for different times followed by the addition of a PDE3 inhibitor, cilostamide (10 μM), up to 24 hours of IVM. Each column represents the mean percentage of GV stage oocyte at 24 hours ± SEM of at least three replicates. The number of oocytes per treatment is indicated under each bar. Statistical analysis by ANOVA showed that the treatments had significant effects (P < 0.0001). Different letters indicate significant differences (P < 0.05) according to the Bonferroni post hoc test. (B) Time-course of resumption of meiosis in porcine COC over 25 hours. Non-linear regression showed that 50% of the oocytes had resumed meiosis at 20.8 hours of culture. For each time point, 60 oocytes were used. Each value represents the mean percentage of GV stage oocytes ± SEM of at least three replicates.