Corneal abnormalities in Pax6+/- and Pax6+/-; Gli3+/- eyes. Corneal morphology of (A) wild-type, (B) Gli3+/-, (C) Pax6+/- and (D) Pax6+/-; Gli3+/- animals. (C) Pax6+/- and (D) Pax6+/-; Gli3+/- eyes have a thinner corneal epithelium and more hypercellular stroma (arrows in C) than wild-type (A) and Gli3+/- (B) eyes. Graphs show relative thicknesses of (E) corneal epithelium and (F) corneal stroma of the various genotype groups. The corneal epithelium of Pax6+/- and Pax6+/-; Gli3+/- eyes was significantly thinner than that of wild-type and Gli3+/-eyes, both centrally and peripherally. The peripheral stroma of Pax6+/-; Gli3+/- eyes was thinner than that of wild-type and Gli3+/- eyes, thus making the mean stromal thickness (average of peripheral and central) of Pax6+/-; Gli3+/- eyes significantly less than that of wild-type, Pax6+/- and Gli3+/- eyes. * = significantly different to wild-type; † = significantly different to Gli3+/-; ‡ = significantly different to Pax6+/-. ce, corneal epithelium; cs, corneal stroma. Scale bar = 50 μm.