Figure 1From: Penetrance of eye defects in mice heterozygous for mutation of Gli3 is enhanced by heterozygous mutation of Pax6(A) Gli3 mRNA expression in the developing eye at E14.5. mRNA expression is observed in the neural retinal, retinal pigment epithelium, lens and surface ectoderm. (B-E) Pax6 protein expression in (B) wild type, (C)Gli3+/-, (D) Pax6+/- and (E) Pax6+/-; Gli3+/- eyes. In wild type embyos (B), Pax6 is expressed in cells of the neural retina, lens epithelium and surface ectoderm. Gli3+/-embryos (C) show identical Pax6 expression to wild type with no overt retinal dysmorphology. Eyes of the developing Pax6+/- embryo (D) show a similar Pax6 expression to wild type but cells forming the persistent lens stalk (arrow) can be clearly seen to be Pax6 expressing. Pax6+/-;Gli3+/- eyes (E) exhibit Pax6 expression localised to the neural retina, retinal pigment epithelium and lens epithelium but show overt distal retinal dysplasia. Rpe, retinal pigment epithelium; nr, neural retina; L, lens; se, surface ectoderm. Scale bar = 200 μm.Back to article page