(A) Gli3 mRNA expression in the developing eye at E14.5. mRNA expression is observed in the neural retinal, retinal pigment epithelium, lens and surface ectoderm. (B-E) Pax6 protein expression in (B) wild type, (C)Gli3+/-, (D) Pax6+/- and (E) Pax6+/-; Gli3+/- eyes. In wild type embyos (B), Pax6 is expressed in cells of the neural retina, lens epithelium and surface ectoderm. Gli3+/-embryos (C) show identical Pax6 expression to wild type with no overt retinal dysmorphology. Eyes of the developing Pax6+/- embryo (D) show a similar Pax6 expression to wild type but cells forming the persistent lens stalk (arrow) can be clearly seen to be Pax6 expressing. Pax6+/-;Gli3+/- eyes (E) exhibit Pax6 expression localised to the neural retina, retinal pigment epithelium and lens epithelium but show overt distal retinal dysplasia. Rpe, retinal pigment epithelium; nr, neural retina; L, lens; se, surface ectoderm. Scale bar = 200 μm.