age-1(mg109) suppressor mutants differentially redistributes DAF-16:GFP localization. Intestinal DAF-16:GFP localization redistributed to the nucleus of wildtype animals after a period of heat stress (35°C) (A, B). DAF-16:GFP localization is primarily in the nucleus of daf-16(mg255);age-1(mg109) animals (C) reflecting localization of DAF-16 in a age-1(mg109) animal. In contrast, DAF-16:GFP localization was seen in both the cytoplasm and nucleus of age-1(mg109);akt-1(mg247) animals (D), age-1(mg109);pdk-1(mg261) animals (E) and age-1(mg109);mg227 (F). Scale bar 50 μm.