Figure 5
The age-1(mg109) stress resistance phenotype is differentially altered by dauer arrest suppressors. (A) Oxidative stress was assessed by treatment with 10 mM paraquat on normal agar medium from the first day of adulthood. age-1(mg109) animals were more resistant to oxidative stress when compared to wildtype animals. Both daf-16 alleles suppressed the age-1(mg109) stress resistant phenotype. Neither akt-1(mg247), pdk-1(mg261) or mg227 suppressed oxidative stress resistance of age-1(mg109). *P < 0.0001, vs. age-1(mg109). Mean survival ± SE, age-1(+), 2.76 ± 0.07 days (n = 96); age-1(mg109), 5.14 ± 0.10 days, (n = 107); daf-16(mg255);age-1(mg109), 4.02 ± 0.09 days, (n = 112); daf-16(mg242);age-1(mg109),3.22 ± 0.05 days, (n = 122); age-1(mg109);akt-1(mg247), 8.6 ± 0.18 days, (n = 112);age-1(mg109);pdk-1(mg261), 5.96 ± 0.32 days, (n = 63);age-1(mg109);mg227, 7.48 ± 0.27 days, (n = 87). (B) Synchronized animal populations were subjected to heat stress at 35°C for 16 hours and scored for survival. Shown is fraction alive in 3 experiments with 60 – 100 animals/experiment; error bars, SEM, #P < 0.05. age-1(mg109) animals were more resistant to heat stress when compared to wildtype animals and both daf-16 alleles suppressed the age-1(mg109) stress resistant phenotype. However, neither akt-1(mg247), pdk-1(mg261) or mg227 suppressed the stress resistance of age-1(mg109). Mean fraction alive ± SD, age-1(+), 0.14 ± 0.14; age-1(mg109), 0.61 ± 0.20; daf-16(mg255);age-1(mg109), 0.39 ± 0.29; daf-16(mg242);age-1(mg109), 0.35 ± 0.43; age-1(mg109);akt-1(mg247), 0.93 ± 0.08; age-1(mg109); pdk-1(mg261), 0.87 ± 0.08; age-1(mg109);mg227, 0.66 ± 0.5.