Table 2 Primers designed for the analysis of WNT4 expression by PCR
From: Differential expression of WNT4 in testicular and ovarian development in a marsupial
Primers | Sequence(5'→3') | Function |
|---|---|---|
R1 | AGCCTGACCATTGGAAGCCCTCT | 5'RACE T-PCR |
R2 | GCCGCACAGAGTCCATCAC | 5'RACE |
F1 | CATCGAGGAGTGCCAGTACCAGTTT | 3'RACE |
F2 | GACGGTGGAACTGCTCGACTCTG | 3'RACE T-PCR |
SMART IV | AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG | 5'RACE |
CDS III | ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30N-1N (N = A, G, C, or T; N-1 = A, G, or C) | 3'RACE |
5' PCR Primer | AAGCAGTGGTATCAACGCAGAGT | 5'RACE |
qF | GAAACCGACGGTGGAAC | qPCR |
qR | AGGAGATGGCATAGACGAA | qPCR |
18S F | GATCCATTGGAGGGCAAGTCT | RT-PCR & qPCR |
18S R | CCAAGATCCAACTACGAGCTTTTT | RT-PCR & Qpcr |
csF1 | GTCATCGGTGGGCAGCATCTC | Cross species cloning |
csR1 | CGTGACACTTGCACTCCACCC | Cross species cloning |