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Figure 7 | BMC Developmental Biology

Figure 7

From: Differential expression of WNT4 in testicular and ovarian development in a marsupial

Figure 7

Immunolocalisation of WNT4 protein during gonadal development in the tammar. The relative developmental stage of the fetus at each time point examined is shown in the top panels. In fetal gonads one day before birth (d25 of pregnancy), WNT4 protein was distributed broadly in the somatic cells of both females (a) and males (g); from 1–3 days after birth WNT4 stained strongly in the presumptive ovary (b) while it decreased dramatically in the developing testis (h) as the seminiferous cords form. By 12–13 days post partum, WNT4 staining was more specific and clearer. In testis, there was strong WNT4 staining in the Leydig cells and the tunica albuginea (i); there was strong staining in the somatic cells and medulla of the ovary (c). There was no immunoreactivity in any of the negative controls: d, e, f, j, k and l (IgG, pre-immune serum). Co = cortex; M = mesonephroi; Me = medulla; Ov = ovary; ST = seminiferous tubule; T = testis; TA = tunica albuginea. Scale bars= 100 μm

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