Figure 10

mCMV infection and SN50 inhibition of canonical NFκB nuclear translocation. With mCMV+SN50 treatment of E15 SMGs (B), there is a notable increase in β-gal (mCMV) staining compared to mCMV infection alone (A). Control E15 + 6 SMGs (C) are at the Terminal Bud Stage with epithelia surrounding distinct lumina; E15 + 6 SN50-treated SMGs (D) are characterized by a modest decrease in epithelia and larger, somewhat dilated lumina. The glandular morphology of CMV + SN50 (F) differs from that seen with SN50 (D) or mCMV infection (E) alone. mCMV-infected E15 + 6 SMGs (E) exhibit peripherally-localized clusters of large, atypical, basophilic cells (arrows) and greatly dilated lumen (*); by contrast, the stroma of the CMV + SN50 E15 + 6 SMGs is almost entirely composed of polygonal cells with darkly staining nuclei and often eosinophilic cytoplasm, the metaplastic cell type typical of E15 + 12 mCMV-infected SMGs (compare to Fig. 9E). Bar: A, B: 50 μm; C-F: 30 μm.