Role of chromosome stability and telomere length in the production of viable cell lines for somatic cell nuclear transfer

Table 2 SCNT embryo development using collagenase digestion cultures.

Animal	Cell Culture	Total Embryos (N)	Day 8 Blastocyst (%)
CATTLE	SKIN COLL	117	36.1 ± 3.4^a
	SKIN COLL P-30	121	10.7 ± 1.0^b
	EAR COLL	94	34.7 ± 3.4^a
	EAR COLL P-30	76	1.4 ± 1.4^b
GAUR	DART COLL	74	0.8 ± 0.8^a
	PUNCH COLL	90	0.9 ± 0.9^a
	EAR COLL	154	11.8 ± 1.3^b

A significant decrease in blastocyst development was observed in reconstructed embryos from short-lived and near-senescent cell lines. These data indicate that cell culture parameters influence embryo developmental competence following SCNT. N = total number of reconstructed embryos (successfully fused couplets) placed in culture. All cultures for SCNT were prepared at P-3 to P-5 unless otherwise stated. ^{a, b} Superscripts within columns denote a significant difference between treatments (p < 0.05).

ISSN: 1471-213X