TY - JOUR AU - Mastromonaco, Gabriela F. AU - Perrault, Steve D. AU - Betts, Dean H. AU - King, W. Allan PY - 2006 DA - 2006/08/09 TI - Role of chromosome stability and telomere length in the production of viable cell lines for somatic cell nuclear transfer JO - BMC Developmental Biology SP - 41 VL - 6 IS - 1 AB - Somatic cell nuclear transfer (SCNT) provides an appealing alternative for the preservation of genetic material in non-domestic and endangered species. An important prerequisite for successful SCNT is the availability of good quality donor cells, as normal embryo development is dependent upon proper reprogramming of the donor genome so that embryonic genes can be appropriately expressed. The characteristics of donor cell lines and their ability to produce embryos by SCNT were evaluated by testing the effects of tissue sample collection (DART biopsy, PUNCH biopsy, post-mortem EAR sample) and culture initiation (explant, collagenase digestion) techniques. SN - 1471-213X UR - https://doi.org/10.1186/1471-213X-6-41 DO - 10.1186/1471-213X-6-41 ID - Mastromonaco2006 ER -