Table 3 PCR primers used.
From: Distinct differentiation characteristics of individual human embryonic stem cell lines
Name | GenBank | Sequence of 5'-primer | Sequence of 3'-primer | Product size |
|---|---|---|---|---|
DAZL | NM_001351 | GGAGCTATGTTGTACCTCC | CCATGTAACTAGATAAGCCAG | 313 bp |
PIWIL2 | BC025995 | TCTATGGGGCCATCAAGAAG | CCATCCCGATCACCATTAAC | 195 bp |
TEX14 | BK000998 | TCCTGTTTTTGGAAGCGACT | GTGGCAGCTGAACAAAGTGA | 214 bp |
STELLA | AY317075 | CACAAATGCTCACCGAAGAA | TTCGATTTCCCTGAGGACTG | 182 bp |
FGF4 | NM_002007 | TCACCGATGAGTGCACGTTCA | GAGGAAGTGGGTGACCTTCAT | 158 bp |
NANOG | AB093576 | GGAAGACAAGGTCCCAGTCA | ATTGTTCCAGGTCTGGTTGC | 349 bp |
OCT4 | XM_084899 | CGTGAAGCTGGAGAAGGAGAAGCTG | AAGGGCCGCAGCTTACACATGTTC | 245 bp |
PUM2 | AF315591 | CCAACATTCCTTGGTGAG | ATCAGGACCCCAAGAAGAGG | 402 bp |
C-ACTIN | NM_005159 | TGATATCCGCAAGGACCTGT | GCTGGAAGGTGGACAGAGAG | 200 bp |
MYOSIN | NM_013292 | TCCATGTTCGACCAGACTCA | AAGCGGTCACACTGCGTGGT | 335 bp |
CMP | NM_002379 | AGAGCTACAGCGTCATCGAG | TCTGACACGTCCAGCGTATC | 316 bp |
NF-L | NM_006158 | CAAAGAGTGAAATGGCACGA | AGCGGGTGGACATCAGATAG | 231 bp |
TUBULIN3β | NM_006086 | CATCCAGAGCAAGAACAGCA | TCGGTGAACTCCATCTCGTC | 234 bp |
ALBUMIN | XM_031320 | GCACAATGAAGTGGGTAACC | CAGCAGTCAGCCATTTCACC | 349 bp |
FOXA2 | NM_021784 | TGCCAGGAGCACAAGCGAGG | TGTTCGTAGGCCTTGAGGTCC | 290 bp |
HNF1α | NM_000545 | CAGGTCTTCACCTCAGACAC | GAGGCCATCTGGGTGGAGAT | 263 bp |
HNF1β | NM_000458 | ACCTTGACGAATATCCACAGC | CTGTGACCACCATTGCAGATG | 364 bp |
HNF6 | U96173 | AGGGCAGATGGAAGAGATCA | TGGATGGACGCTTATTTTCC | 377 bp |
PDX1 | U30329 | ACCAAAGCTCACGCGTGGAA | CTCTCGGTCAAGTTCAACAT | 191 bp |
GAPDH | M33197 | GTCTTCACCACCATGGAGAAGGCT | TGTAGCCCAGGATGCCCTTGAGGG | 529 bp |