Figure 3

The fibroblast response to IGF-I and IGF-II. The MTT assay demonstrated that all populations of fibroblasts responded to IGF treatment with statistically significant (p < 0.001) greater cell numbers. In this representative experiment the mean and SEM data represents the pooled data from duplicate samples from the three cell lines. Maternal fibroblasts responded with greater cell numbers for IGF-I than IGF-II compared to controls (88% vs 55% increase,* p < 0.001). Fetal fibroblasts responded to IGF-II better than IGF-I, although not significantly, and at the same rate as maternal fibroblasts responded to IGF-I (88% vs 79%, p = 0.34). The amount of increase in the fetal cells in response to IGF-II was significantly greater that the amount of increase seen in the maternal cells (^p < 0.001). Error bars are standard error of the mean calculated from the original absorbance data and divided by the mean absorbance of the controls to create a percent value.