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Figure 5 | BMC Developmental Biology

Figure 5

From: Rho-kinase regulates tissue morphogenesis via non-muscle myosin and LIM-kinase during Drosophiladevelopment

Figure 5

DRok in axonal development. (A, B) Immunostaining of either wild-type (A) or DRok-cat-expressing (B) photoreceptor neurons which send axonal projections from the developing 3rd instar larval eye disc into the optic lobe of the brain. Axonal guidance and targeting appear normal in DRok-cat-expressing larval eye discs. GMR-Drok-cat/+ photoreceptors project correctly to the lamina and medulla layers into the optic lobe. In B, the axons are folded due to tissue mounting. (C-H) Double immunostaining of either wild-type (C-E), or GMR-Drok-cat/+ (F-H) 3rd instar larval eye discs, with phalloidin (C, F) and an anti-Elav antibody (D, G) to detect actin and differentiating neurons, respectively. The overall morphology and differentiation pattern in photoreceptors is undistinguishable between wild-type and DRok-cat-expressing 3rd instar larval eye discs. (I-L) Scanning electron microscopy pictures of wild-type (I), GMR-Dlimk/+ (J), GMR-Drok-cat/+ (K) or GMR-Dlimk/GMR-Drok-cat (L) eyes. Whereas overexpression of DLimk or expression of DRok-cat, separately, does not perturb the external morphology of the eye, co-expression of DLimk and DRok-cat results in a strong rough eye phenotype associated with decreased eye size. (M-P) Immunostaining of the embryonic CNS (BP102 antibody) of the following genotypes: wild-type (M), elav-Gal4>UAS-Drok-cat (N), elav-Gal4>UAS-Dlimk (O) or elav-Gal4>UAS-Drok-cat, UAS-Dlimk (P). As observed for the eyes, whereas overexpression of DLimk or expression of DRok-cat alone does not alter the proper organization of the embryonic CNS marked by adjacent patterns of connected neurons (M), co-expression of DLimk and DRok-cat leads to the disruption of connecting neurons (P, arrow).

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