Figure 3

Genetic interactions between Drok and several genes involved in Rho signaling pathways. (A-D) Tangential retinal sections of eyes of the following genotypes: wild-type (A), one-copy GMR-Drok-cat transgenic (B), or one-copy GMR-Drok-cat and one mutant loss-of-function allele of either adducin (hts1103) or diaphanous (dia) (C or D, respectively). Taking away one copy of either adducin or diaphanous reverts the GMR-Drok-cat-induced eye phenotype to a near to wild-type appearance. (E-H) Light microscopy photographs of a en-GAL4<UAS-Drok-cat expressing wing on its own (E) or en-GAL4<UAS-Drok-cat expressing wings in various heterozygous loss-of-function mutant backgrounds including hts1103 or dia or rho1⁷²⁰ (F-H). Taking away one copy of adducin (hts1103) does not rescue the missing crossvein phenotype, whereas heterozygosity for one copy of diaphanous (dia), or for one copy of rho1 (rho1⁷²⁰) almost entirely rescues or partially rescues the wing phenotype, respectively.