Skip to main content
Figure 5 | BMC Developmental Biology

Figure 5

From: Alterations in gene expression in T1α null lung: a model of deficient alveolar sac development

Figure 5

Comparative expression analyses of mRNA and protein levels of EphrinA3 and Ptgs1. Representative western blot and densitometry analysis of (A) EphrinA3 and (B) Ptgs 1 expression in total lung protein extracts (50 μg) at E18.5. Protein expression data are compared to the respective mRNA level determined by microarrays and QRT-PCR. Western blot data were normalized to the densitometry value of the Coomasie blue stained gels and total protein loaded. (+/+) indicates wild type, and (-/-) null genotypes respectively. n = 5 (E18.5) or 3 (term) for microarrays, n = 3 for QRT-PCR, and for western blot analyses for each genotype. Error bars represent standard error of the mean. (*) indicates p ≤ 0.05, (**) indicates p ≤ 0.01, (#) indicates p = 0.056.

Back to article page