Figure 4

Real time RT-PCR validation of selected genes identified by microarray analyses in E18.5 and term T1α null lungs. A. Expression levels obtained by microarray analysis of RNA from E18.5 wild type (+/+) lungs (relative value = 1, black bars) compared to E18.5 T1α (-/-) lungs (fold change relative to wild type, dotted bars) (n = 5). B. Real time RT-PCR validation of the genes depicted in A (n = 3-6). C. Expression levels obtained in the microarray analysis of RNA from term wild type (+/+) lungs (relative value = 1, black bars) compared to term T1α (-/-) lungs (fold change relative to wild type, hatched bars) (n = 3). D. Real time RT-PCR validation of the genes depicted in C. Data is normalized to actin expression level. (n = 3-6). Error bars represent standard error of the mean. (*) indicates p ≤ 0.05; (**) indicates p ≤ 0.01; (#) indicates p = 0.056.