Figure 3

p21 and PCNA expression is altered at term but not at E18.5 in T1α null lungs. (A) Representative western blot and densitometry analysis of PCNA in total lung protein extracts (10 μg) at E18.5 and term, (B) densitometry analysis of p21 northern blots at term, (C) representative western blot and densitometry analysis of p21 in total lung protein extracts (10 μg) at E18.5 and term, and (D) representative blots of p21, p27 and p57 mRNA in total lung RNA at term (10 μg) normalized to β-actin. Time points analyzed were E18.5 and term. (+/+) indicates wild type, (+/-) heterozygous, and (-/-) homozygous genotypes respectively. n = 3 of each time point and genotype. Bars represent mean. Error bars represent Standard Deviation. (*) indicates p ≤ 0.05; (#) indicates p = 0.059.