Figure 1

Expression of T1α protein on the apical surface of mouse lung epithelial cells during development. Immunohistochemistry analysis of T1α protein using mAb 8.1.1 antibody in mouse lung paraffin sections. (A) At E11.5 T1α is expressed on the apical surface of epithelial cells in the forming lung bud, (B) at E15.5 it is expressed in the cuboidal epithelial cells of the distal lung, (C) at E18.5 alveolar epithelial cells are starting to flatten and T1α antibody labels the apical surface of the alveolar walls, and (D) at postnatal day 1, type I cells express T1α protein while type II cells are not labeled. Red arrows indicate T1α expression. Black arrowheads indicate unstained type II cells at late gestation. Bar = 25 μm