Figure 7

DNA methylation states of the Octamer/Sox elements of non-ECAT genes. A. Bisulfite genomic sequencing was performed to examine the DNA methylation profiles for individual CpG sites in the Octamer/Sox elements of non-ECAT genes in ES cells, somatic cells, and male germ cells. Results are shown as described in Figure 2 and 3. Oblique lines indicate regions not examined and their approximate lengths are shown. B. Results of RT-PCR showing the expression of REST/NRSF, Rif1, and Tcf3 in ES cells and GS cells.