Figure 3

DNA methylation states of non-ECAT genes. Bisulfite genomic sequencing was performed to examine the DNA methylation profiles for individual CpG sites in the promoter regions of germ cell specific genes Mvh and preproacrosin, and a differentiation marker Hoxb1 in ES cells, somatic cells, and male germ cells. Results are shown as described in Figure 2. In the diagram for the Hoxb1 gene, the open square and shaded circle indicate the binding sites for Sox2 and Oct1, respectively.