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Figure 2 | BMC Developmental Biology

Figure 2

From: Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells

Figure 2

DNA methylation states of ECATs expressed in GS cells. Bisulfite genomic sequencing was performed to examine the DNA methylation profiles for the individual CpG sites of ECAT genes in ES cells, somatic cells, and male germ cells. Schematic diagrams of regions analyzed are shown in the upper part of the figure. Black rectangles represent the exons, and arrows indicate transcription initiation sites. Black horizontal bars indicate the regions analyzed. All of diagrams are in scale relative to a 100-bp scale bar. In the diagram for the Rex1 gene, the shaded square and open circle indicate the binding sites for Rox1 and Oct3/4, respectively. DNA methylation states in ES cells, somatic cells, and germ cells are shown in the lower part of the figure. Open circles indicate unmethylated CpGs and closed circles indicate methylated CpGs. The numbers indicate the relative positions of CpG sites from the transcription initiation site. The CpG nearest to the transcription initiation site is described as -1 (upstream) or 1 (downstream). Positions where CpG is absent due to DNA polymorphism are indicated by hyphens. ES, undifferentiated ES cells (129 background); ES (RA), ES cells differentiated by retinoic acid treatment; MEFs, MEFs derived from E13.5 C57BL/6J embryos; GS, GS cells derived from DBA neonate testis. Brain, kidney, and testis were obtained from 8-week-old C57BL/6J mice. Sperm was isolated from the epididymis of 11-week-old C57BL/6J mice.

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