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Figure 10 | BMC Developmental Biology

Figure 10

From: Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells

Figure 10

The binding of Oct3/4 and Sox2 to the Octamer/Sox elements. A. ChIP analyses was performed with anti-Oct3/4 antibody (αOct3/4) and anti-Sox2 antiserum (αSox2), or with normal mouse IgG as a negative control (IgG). The genomic region with the Octamer/Sox element in Nanog, Fgf4, Fbx15, Sox2, UTF1, and Rif1 gene was amplified with specific primers. B. Western blot analysis of Oct3/4 and Sox2 proteins in ES cells and GS cells. We used β-actin as a loading control.

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