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Table 3 Inhibition of Ca2+-uptake activity. The purified CV-fraction P0E and the purified ER-fraction P1E were tested for sensitivity of Ca2+-uptake to Tg (22 μM), BHQ (200 μM), CMA (40 μM) and Sr2+ (30–60 μM) as indicated. nd: not determined. Data are means ± SD. The number of independent experiments is given in brackets.

From: The contractile vacuole in Ca2+-regulation in Dictyostelium: its essential function for cAMP-induced Ca2+-influx

Fraction

Tg %

BHQ %

CMA %

Sr2+ %

P0E (CV)

nd

25 ± 13 (3)

57 ± 6 (4)

52 ± 13 (3)

P1E (ER)

45 ± 23 (4)

72 ± 4 (3)

nd

43 ± 6 (4)