Table 2 Purification of CV and ER fractions by antibody. Dajumin-GFP labeled CV present in P0 were bound to anti-GFP-microbeads and separated from the unbound vesicles in a magnetic field as detailed in Methods. P0F represents the flow through, P0E is the eluate containing CV. Calnexin-GFP labeled ER present in P1E was purified in the same way. P1F: flow through, P1E: eluate containing ER. As described in Methods, the measured values of Ca2+-uptake were corrected for the same initial [Ca2+]ev. Data are means ± SD. The number of independent experiments is given in brackets.
Fraction | GFP-label % | Ca2+-uptake % | Specific activity of Ca2+-ATPase (%) |
|---|---|---|---|
P0F (Dajumin) | 12.4 ± 3.9 (3) | 15.6 ± 9.7 (3) | 18 ± 5.4 (3) |
P0E (Dajumin) | 87.6 ± 3.9 (3) | 84.4 ± 9.7 (3) | 109 ± 9.3 (3) |
P1F (Calnexin) | 13.8 ± 4.2 (4) | 13.4 ± 8.3 (4) | 8.1 ± 6.3 (4) |
P1E (Calnexin) | 86.2 ± 4.2 (4) | 86.6 ± 8.3 (4) | 87 ± 66 (4) |