Table 1 Characterization of crude vesicular fractions. Vesicles were obtained by differential centrifugation of lysed cells as described in Methods. The amount (percent) of CV (± S.D.) in each fraction was determined according to the dajumin-GFP label present, while that of the ER by the presence of calnexin-GFP label. Total Ca2+-transport activity obtained from 4 × 108 cells is shown for each fraction. Data are means ± SD. The number of independent experiments is given in brackets.
Fraction | Sedimentation | CV | ER | Ca2+-uptake | |
|---|---|---|---|---|---|
(g) | % | % | nmol | % | |
P0 | 3.800 | 42.4 ± 4.0 (4) | 33.5 ± 3.7 (3) | 205 ± 42 (3) | 67 |
P1 | 12.000 | 33.6 ± 7.2 (4) | 43.0 ± 2.8 (3) | 76 ± 24 (3) | 25 |
P2 | 40.000 | 24.0 ± 10.6 (4) | 23.5 ± 1.3 (3) | 25 ± 6 (3) | 8 |