Figure 3
Ca2+-uptake and Ca2+-release in P 0 E. P0E was obtainedby GFP-antibody purification. Ca2+-uptake was induced by 0.4 mM ATP and measured with Fluo-3 as described in Methods. A Ca2+-calibration pulse was followed by further Ca2+-uptake. W-7 and AA induced Ca2+-release, 4-BrA23187 elicited further Ca2+-release. One out of three independent experiments is shown.