Table 1 Microsatellite loci used in this study; repeat motif, allele size range in base pairs, and nucleotide sequence (all from [24]).
From: Assigning sex to pre-adult stalk-eyed flies using genital disc morphology and X chromosome zygosity
Locus | Repeat Motif | Product Size (bp) | Primer Sequence |
|---|---|---|---|
ms-054 | [AC]2ATTAT[AC]10AT[AC]1 | 165 | F:FAM-ACGGAAGTAACACAAAAAGAT R:TCAGCGCTACTCACAGAACTAACT |
ms-125 | [GT]14 | 153 | F:FAM-TGGTGTTAATGAACGAGTGACTTC R:TGCCATTCATGCAAGTCTTC |
ms-167 | [AC]1TC[AC]9 | 222 | F:FAM-GCTGCGAGCTGTAAAACAGA R:GGCAGTGACAATGGCAGTAA |
ms-395 | [GT]10AT[GT]1 | 200 | F:HEX-CGAGTAGAGCACTTTGAAGATACA R:TTGCGGTTGTAGAAGTTTGC |