Figure 8

wdr68 activity is functionally conserved. A) Sequence similarity scores generated using ClustalW between wdr68 homologs among selected representative organisms. Region shown contains the functionally important position, highlighted in red, identified in the ttg1-9 allele. B) The T284F mutant cannot rescue the dys mutant phenotype. Live 4dpf animal shown. C) The drosophila homolog CG14614 rescues the dys mutant phenotype as efficiently as the zebrafish transcripts. D) Alcian blue staining of attempted rescue animals using T284F transcripts. E) Alcian blue staining of animals rescued using the drosophila homolog CG14614. F) Chart summarizing the results of a rescue experiment showing that zebrafish wdr68 transcripts rescue the strong mutant phenotype, that T284F transcripts fail to rescue, and that CG14614 rescues as efficiently as zebrafish wdr68.