Figure 3

Expression pattern for the wdr68 gene during early development. A) Transcripts are present in all cells of the animal at sphere stage. B) Ubiquitous expression at tailbud stage. C) Ubiquitous expression at 5 somites stage. D) Ubiquitous expression at 10 somites stage. E) Ubiquitous expression with modest enrichment in the forebrain and hindbrain at 20 somites stage. Also note emerging patterned expression in the developing somites. F) Dorsal view of same 20 somites stage animal indicating ubiquitous expression. G) Ubiquitous expression with moderate enrichment in the head region at 24 hpf. H) Higher magnification image of a 28 hpf animal to show patterned expression in somites. I, J) Wild type sibling and dys/dys homozygous mutant at 34 hpf. I) Enriched expression in the head region with much lower expression in somites in a 34 hpf wild type sibling animal. J) Absence of detectable wdr68 transcripts in the dys/dys homozygous mutant sibling. K) Dorsal view of head region of 34 hpf animal showing near ubiquitous expression in developing head structures.