Figure 2

The dys^hi3812 mutant is a null. A) Diagram indicating the location of the dys^hi3812 proviral insert in the first exon of the wdr68 gene. B) RT-PCR analysis of wild type siblings and dys/dys animals showing that wild type wdr68 transcripts are not detectable in the dys mutants indicating that the mutant is a null allele. C) RT-PCR analysis reveals a maternal supply of wdr68 transcripts. cDNA from wild type embryos at zero = unfertilized, 3 hpf, 6 hpf, 10 hpf, and 19 hpf. As negative control, edn1 transcripts are only detected after 10 hpf. Transcripts for the ribosomal gene rpL35 are present at all stages examined.