Figure 8

Noggin and Dkk-1 regulate the expression of newly identified genes. 4-cell embryos were ventrally injected with the noggin and/or dkk-1 concentrations described in Figure 1, and tested by in situ hybridization for patterns of ectopic induction or repression. In each case the observed in situ patterns confirm the microarray patterns (A-D) LRIG2 shows ectopic ventral expression in both the Noggin and the Noggin+Dkk-1 overexpressing embryos, but not in the Dkk-1 embryos. (E-H) ARHGEF3 shows ectopic ventral expression only in the Noggin+Dkk-1 overexpressing embryos. (I-L) HES6 shows ectopic ventral repression in the Noggin+Dkk-1 and the Dkk-1 overexpressing embryos. (M-P) Frzb3 expression is ectopically repressed by Noggin or Noggin+Dkk-1 overexpression. (Q-T) Xl.3529.1.A1 expression is ectopically repressed by Noggin+Dkk-1 and Dkk-1 overexpression. (U-X) Gadd45g is ectopically induced only in the Noggin+Dkk-1 condition. All embryos are between stages 10.5 and 11 and are shown in vegetal view with dorsal side facing up.