Figure 6

Clusters 3 and 4 faithfully predict expression patterns for unknown genes. Genes found in clusters 3 and 4 that lacked described gastrula stage expression patterns were analyzed by whole mount in situ hybridization. Unknown genes from cluster 3 that showed a specific pattern are enriched in organizer tissues (orange box), and unknown genes from cluster 4 that showed a specific pattern are excluded from organizer tissues (magenta box). Each tested gene is labeled with its name and the Affymetrix probe set number. Genes marked "no pattern" showed no staining, or a non-specific staining pattern that was similar to sense controls. Genes marked "no gastrula pattern," showed no pattern during gastrula stages, but did show specific patterns at later stages that are not shown here. Each photo is labeled with the developmental stage of the embryo in the bottom left corner, and the orientation in the bottom right corner. veg: vegetal view, dorsal faces up. dor: dorsal view, anterior faces up.