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Figure 2 | BMC Developmental Biology

Figure 2

From: Positive autoregulation of the transcription factor Pax6 in response to increased levels of either of its major isoforms, Pax6 or Pax6(5a), in cultured cells

Figure 2

Quantification of Pax6 and Pax6(5a) in over-expressing cell lines. (A) Western blots showing Pax6(5a) and Pax6 production in four over-expressing stably transfected lines (20 mg protein per lane). In control lines, Pax6 proteins were not detectable unless 100 mg of protein were loaded per lane. (B) Quantification by densitometry of over-expression of Pax6(5a) and Pax6 in four over-expressing cell lines: lines 1 and 2 contained Pax6(5a) constructs, lines 3 and 4 contained Pax6 constructs. All values were expressed relative to levels of Pax6 in a control cell line. Mean values ± SEMs are plotted for levels of Pax6(5a) (grey bars) and Pax6 (black bars); all values, given below the histograms, are significantly higher than in controls (unpaired Student's t-tests: p < 0.01).

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