Figure 3
From: RCAS-RNAi: A loss-of-function method for the developing chick retina
Test of RCAS-RNAi activity on Dio2 protein activity and transcript expression
(A) Infection of chick retina with RCAS-U6-Dio2 and biochemical analysis of Dio2 activity. Stage 10 retinas were injected with RCAS-U6-Vis1, RCAS-U6-Dio2, or left uninfected, and RPE tissue was harvested at approximately E6. Dio2 activity in the RPE was assayed by measuring free I125 production.
(B) Infection of chick retina with RCAS-U6-Dio2 and analysis of Dio2 and visinin expression. Stage 10 retinas were injected with RCAS-U6-Dio2 and harvested at approximately E6. Dio2 and visinin transcript expression were assayed by in situ hybridization, followed by viral antigen detection using 3C2 (anti-gag) antibody.