Figure 1

RNAi construct directed against cnbA. Fragments of cnbA were generated by PCR using oligonucleotide primers flanked by suitable restriction sites. The short and long fragments were amplified with primers 1 and 2 or primers 3 and 4, respectively. They were then fused in opposite orientation and cloned in pDNeoII. See Materials and Methods for further details. P, actin6 promotor.