High resolution ultrasound-guided microinjection for interventional studies of early embryonic and placental development in vivoin mice

Table 1 Target accuracy following micro-injection.

Age	Target	Necropsy	Mice	Concepti¹	Beads in conceptus²		Beads localized in target³
			#	#	#	%	#	%	95% CI
E6.5	EPC	Within 24 hours	5	55	50	91	28	51*	38–64
E7.5	EPC	Day of injection	4	40	38	95	36	90	77–96
E7.5	AC	Day of injection	4	50	47	94	34	68	54–79
E7.5	Exo	Day of injection	4	43	40	95	35	81	68–91

1 number of concepti analyzed (<10% were damaged during dissection and were not included)
2 number of concepti containing beads at one or more sites
3 number of concepti with beads localized in target with few or no beads off-target
* significantly different than EPC at E7.5
EPC = ectoplacental cone region, AC = amniotic cavity, Exo = exocoelomic cavity, CI = confidence interval

ISSN: 1471-213X