Figure 4

2D gels. 2D gel electrophoresis of samples derived from embryos at 80% epiboly (8 hpf) in the acidic as well as in the basic range. A. pI 4–7 (24 cm), fluorescently labelled with Cy2, 50 μg protein/dye. Spots processed for MS-identification are labelled (ID of Additional file 1). B. pI 6–9 (18 cm), silver stain.